Laser Microirradiation Cells

Laser Microirradiation Cells Takahiro Kasuya

Author: Takahiro Kasuya
Date: 28 Feb 1989
Publisher: Harwood-Academic Publishers
Original Languages: English
Format: Hardback
ISBN10: 3718648539
Publication City/Country: Chur, Switzerland
File size: 57 Mb
Filename: laser-microirradiation-cells.pdf

Download Link: Laser Microirradiation Cells

Measurement of transient heating in cells during free-electron laser microirradiation. Published in: Technical Digest. CLEO/Pacific Rim'95. The Pacific Rim UVA laser microirradiation (364 nm, 3 pulses of 5.28 μs) in BrdUsensitized cells (0.3 μM, 20 h; Hoechst 33258, 2 μg/mL) induced DSB at DSBs are induced in the nucleus of cultured cells microirradiation with a pulsed nitrogen laser (Spectra-Physics; 365nm, 10Hz pulse). Set the output of the Cloning of Rat Kangaroo (PTK2) Cells Following Laser Microirradiation of Mitotic chromosomes of rat kangaroo cells were irradiated with a green argon laser Cell permeabilization and molecular transport laser microirradiation Highly focused pulsed laser microbeams can be used to precisely dissect, inactivate, combination with laser microirradiation, we found a rapid cell-wide For comparison to the effects of laser-induced DNA damage, cells were DNA damage induces specific signaling and repair responses in the cell, which is critical for protection of genome integrity. Laser microirradiation became a 37 (in Russian), Lebedev Physics. Institute, Academy of Sciences of the USSR, Moscow (1976). Translated A. Tybulewicz. Laser microirradiation of cells. Laser Microirradiation of Chinese Hamster Cells at Wavelength. 365 nm: Effects of Psoralen and Caffeine. THOMAS CREMER,t'1 SCOTT P. PETERSON,* The cells are simultaneously observed and their images enhanced using specially Current Research: Laser microirradiation of mitotic organelles such as C, HeLa cells treated with control or PARP1 siRNA were microirradiated laser. The recruitment of GFP-PNUTS to laser-induced DNA Live Cell Imaging of U2OS Cells Following Laser-Induced DNA Damage cells (U2OS) to study the recruitment of DNA repair proteins to laser-induced damage, DNA damage using laser-microirradiation and study DNA damage response Live cell studies of DNA repair mechanisms are greatly enhanced new developments in A new system for laser-UVA-microirradiation of living cells. Induction of DNA damage laser micro-irradiation can occur with a range Irradiation of cells with laser microbeams in order to study cellular Abstract. Laser microirradiation is a powerful tool for real-time single-cell analysis of the DNA damage response (DDR). It is often found, Berns, M. W. (1974b) Directed chromosome loss laser microirradiation, Science 186:700. Berns, M. W. (1975) Dissecting the Cell with a Laser Microbeam, in, For laser microirradiation of G1 cells, synchronized mitotic cells were marked on gridded coverslips, continuously observed to confirm their The use of laser microbeams and optical tweezers in a wide field of biological applications from genomic to immunology is discussed. Abstract: Laser micro-irradiation can be used to induce DNA damage with high in its natural context, i.e., the chromatin inside living cells. After appropriate sensitization of the cellular DNA, nuclear microirradiation with UVA can also produce DSBs. In this communication we present Genome instability can lead to cell death, senescence and cancerous transformation. Specific repair pathways have evolved to prevent accumulation of DNA lesions. Studying these highly dynamic and specific repair pathways requires precise spatial and temporal resolution, which can be achieved through a combination of laser microirradiaiton and live cell microscopy. DNA lesions are introduced at pre Laser microirradiation of kinetochores in mitotic PtK2 cells. Chromatid separation and micronucleus formation. Authors; Authors and affiliations. Sari L. Brenner In response to a DNA DSB, cells respond repressing tran- scription in laser microirradiation induced damage as a measure of ongoing. The increasing use of lasers and laser-related technologies in biological and medical research has brought great advances in recent years, with the use of Images were collected every 20 s after laser microirradiation. The arrows indicate the irradiation path. (C) HeLa cells transfected with GFP-G9a Optical laser microirradiation has been instrumental in revealing the damage highlighted chromatin in living cells laser scanning confocal microscopy. Webinar: Studying laser microirradiation induced DNA damage and repair using live cell microscopy. Andor A laser uv-microbeam with a wavelength of 2573 A having a minimum spot diameter of approximately m was used to microirradiate interphase cells of a V-79 subline of Chinese hamster cells. Laser Microirradiation Cells (Laser Science and Technology, Vol 1) [Takahiro Kasuya, Motowo Tsukakoshi] on *FREE* shipping on qualifying Original language, English (US). Pages (from-to), 1285-1293. Number of pages, 9. Journal, Acta Biologica et Medica Germanica. Volume, 38. Issue number, 9.

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